||Aim: to evaluate the total amount of phenolic compounds and flavonoids and also to analyse antioxidant activity and iron chelating activity of Astragalus glycyphyllos L. dried materials and to predict possible use of analysed plant. The objectives of the research: to evaluate the total amount of flavonoids and total amount of phenolic combinations in the samples A. Glycyphyllos; to analyse the antioxidant activity of the herbal materials; to analyse iron chelating activity of the herbal materials; according to the biologically active compounds predict therapeutic applications for cardiovascular diseases. Objects and methods of the assay: the object of this assay is Astragalus glycyphyllos L. material collected in Kėdainiai, Lithuania. Phenolic compounds evaluated according to calibration curve of galic acid, antioxidant activity free DPPH radical inhibition. Iron chelating properties - photometric determination of binded iron. Conclusions: In spectrophotometric analysis, the highest amount of phenolic compounds were determined in Astragalus glycyphyllos leaves (25,22±0,59 mg/g) and flowers (21,6±0,49 mg/g), lowest - in stems. The highest amount of flavonoids were determined in leaves (20,38±0,31 mg/g), lowest in stems (6,12±0,46 mg/g). Flavonoids are the largest group of phenolic compounds in Astragalus glycyphyllos materials. The strongest antioxidant activity was observed in flowers (13,26±0,66%), and the highest antioxidant activity by iron binding properties was determined in flowers (91,97±1,26%), lowest antioxidant activity was observed in stems (61±1,53%). Antioxidant activity of materials are well correlated with phenolic compounds and flavonoids. While comparing the influence of drying method on yield of phenolics, it was determined that liophylization was better than drying usual way.