||The aim to evaluate HLA antibodies laboratory testing in recipients placed on kidney transplant waiting list. Methods and the study subjects. Recipients placed on a waiting list for kidney transplant and consulted at LSMU hospital Kaunas clinics during 2011–2016 were included in the study. Screening and identification of HLA antibodies were performed (LIFECODES LifeScreen Deluxe, LIFECODES LSA Class I/Class II (Immucor, JAV)) using Luminex® technology. Lab identification tests (LSA) were used for PRA evaluation, OPTN calculator was used for calculated PRA (cPRA) values. Results and conclusions. Out of 424 recipients positive screening results of class I HLA antibodies were reported in 132 (72.9%) recipients, and the class II – in 158 (87.3%) recipients. HLA antibodies identification tests results showed that 16.9% recipients awaiting for kidney transplantation are sensitized to class I HLA and 20.9% – to class II HLA. Class I HLA antibodies frequency in women was significantly higher than men (χ2=11.139, ll=1, p=0.001), class II HLA antibodies frequency didn‘t differ significantly between genders. The strength of sensitization to calss I HLA (evaluated by average fluorescence intensity of HLA antibody reactions) in female recipients was higher than in male recipient’s group (weak reactions – p=0.022; moderate reactions – p<0.001; strong reactions – p=0.001). Sensitization to both classes of HLA didn‘t depend on recipient's blood group. Assessing kidney recipient sensitization based on PRA, it was found that the PRA results depended on the evaluation method – results set by calculation method (cPRA) were significant higher compared to the results obtained by laboratory identification of HLA antibodies (LSA) (respectively, class I HLA PRA –Z=-6.058, p<0.001; class II HLA PRA – Z=-7.770, p<0.001).