||Introduction: Operating room nurses’ job satisfaction in Lithuanian health care institutions is little examined area. Nursing shortage becomes a global problem in almost all European countries including Lithuania. Much broader market perspectives have opened for nurses as Lithuania joined the European Union. Objective: To evaluate operating room nurses’ job satisfaction. Specific aims: To investigate operating room nurses’ job satisfaction, to determine factors that cause job satisfaction and to make recommendations to increase job satisfaction. Methods: Single-step anonymous questionnaire survey for operating room nurses. The statistical analysis performed using SPSS 17.0 program. MS Excel 2007 was used for graphical depiction of the test results. Results: Most of the operating room nurses are satisfied with their job (82.9%) and more than two-thirds (70.7%) of respondents would choose nursing career again. The majority (78.9%) of operating room nurses are satisfied with the work schedule. Two-thirds satisfied with the quality of operating room management (69.9%), salary (69.2%), workload (66.7%), opportunity of training and qualification improvement (66.7%). More than a half of operating nurses (60.2%) are satisfied with teamwork and organization of work in operating room (51.2%). Only a little bit more than a third of respondents (38.2%) are satisfied with doctors’ attitude towards operating room nurses and relationship among employee (37.4%). Higher salary (63.4%), good teamwork (18.9%) and treating nurse as a member of teamwork (12.0%) are the encouraging factors for operating room nurses to work better. Conclusions: Most of the operating nurses are satisfied with their job (82.9%), work schedule, workload, quality of operating room management and opportunity for training and improvement. Higher salary, good teamwork and treating nurse as a member of teamwork are the encouraging factors for operating room nurses for better work. Recommendations: Quality of service provided by operating room nurses could be achieved encouraging teamwork collaboration, increasing prestige of the nursing, encouraging nurses for training and gaining higher education, improving salary system.