Title Flavonoidų, kaip citochromą c redukuojančių junginių, tyrimas
Translation of Title Evaluation of flavonoids as compounds reducing cytochrome c
Authors Eimontas, Tadas
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Pages 47
Keywords [eng] Flavonoids ; cytochrome c ; apoptosis
Abstract [eng] Aim. To test ability of flavonoids, quercetin, myricetin, kaempferol and their glycosides to reduce cytochrome C. Tasks. 1) To evaluate ability of analysed flavonoids to reduce cytochrome c based on the amount of hydroxyl groups in the ring B of the structure. 2) To evaluate the effect of sugar-part of the analysed flavonoids structure on ability to reduce cytochrome C. 3) To evaluate the results and to compare ability of analysed flavonoids to reduce cytochrome c. Research methods. This study was aimed to tests the effect of quercetin, myricetin, kaempferol and their glycosides (10 µM and 20 µM) on cytochrome c redox status in vitro. The spectrophotometry method was applied. The absorption spectre was measured at the wave length interval of 500-600 nm. The maximal value of the cytochrome c absorption was observed at the 550nm wave length. The results were recorded at the 0, 3, 6, 9, 12, 15, 18 and 21 min of the test. Standard reducer – dithionite (its reduced cytochrome c value was considered as 100% during the calculations). Results. The following results were obtained regarding ability of flavonoids to reduce cytochrome C at the level of 10 µM (results of the 21st min): myricetin - 74.2 ± 5.3%, myricitrin – 59.9 ± 4.5%, quercetin – 52.4 ± 3.1%, kaempferol – 43.5 ± 2.9%, isoquercitrin – 41.7 ± 3.1%, rutin – 39.6 ± 1.7%, quercitrin – 38.3 ± 2.7%, hyperoside – 36.2 ± 2.1% and astragalin – 30 ± 6.6%. The following results were obtained 20 µM level: myricetin – 80.2 ± 7.2%, myricitrin – 65.5 ± 8.9%, quercetin – 61.7 ± 3%, kaempferol – 51.9 ± 4.9%, astragalin - 39.7 ± 1.7%, quercitrin - 40.3 ± 6%, rutin - 37 ± 1%, hyperoside – 35.9 ± 1.8%, isoquercitrin - 35.9 ± 5.8%. Conclusion: Studies revealed that the larger number of hydroxyl groups connected to the ring B of the flavonoid structure is linked to the stronger reducing power of cytochrome c. Also it was revealed that hydroxyl group connection to the position 5 of the ring B of the flavonoid structure causes stronger reducing power than connection to the positions 3 or 4. Also it was revealed that connection of any sugar-part to the flavonoid structure weakens its reducing power.
Dissertation Institution Lietuvos sveikatos mokslų universitetas
Type Master‘s Thesis
Language Lithuanian
Publication date 2016